3-Methyleneoxindole: an affinity label of glutathione S-transferase pi which targets tryptophan 38.

@article{Pettigrew20013MethyleneoxindoleAA,
  title={3-Methyleneoxindole: an affinity label of glutathione S-transferase pi which targets tryptophan 38.},
  author={N. E. Pettigrew and E. Brush and R. Colman},
  journal={Biochemistry},
  year={2001},
  volume={40 25},
  pages={
          7549-58
        }
}
The compound 3-methyleneoxindole (MOI), a photooxidation product of the plant auxin indole-3-acetic acid, functions as an affinity label of the dimeric pi class glutathione S-transferase (GST) isolated from pig lung. MOI inactivates the enzyme to a limit of 14% activity. The k for inactivation by MOI is decreased 20-fold by S-hexylglutathione but only 2-fold by S-methylglutathione, suggesting that MOI does not react entirely within the glutathione site. The striking protection against… Expand
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