3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation

@inproceedings{Connor20093Methyl1butanolPI,
  title={3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation},
  author={Michael R. Connor and Anthony F. Cann and James C. Liao},
  booktitle={Applied Microbiology and Biotechnology},
  year={2009}
}
Interest in producing biofuels from renewable sources has escalated due to energy and environmental concerns. Recently, the production of higher chain alcohols from 2-keto acid pathways has shown significant progress. In this paper, we demonstrate a mutagenesis approach in developing a strain of Escherichia coli for the production of 3-methyl-1-butanol by leveraging selective pressure toward l-leucine biosynthesis and screening for increased alcohol production. Random mutagenesis and selection… CONTINUE READING