3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase of Streptomyces aureofaciens Tü 24. I. Partial purification and properties.

@article{Gorisch19713DeoxyDarabinoheptulosonate7phosph,
  title={3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase of Streptomyces aureofaciens T{\"u} 24. I. Partial purification and properties.},
  author={Helmut Görisch and Franz Lingens},
  journal={Biochimica et biophysica acta},
  year={1971},
  volume={242 3},
  pages={
          617-29
        }
}

The kinetics of a purified form of 3-deoxy-D-arabino heptulosonate-7-phosphate synthase (tryptophan) from Neurospora crassa.

  • K. IpC. H. Doy
  • Biology, Computer Science
    European journal of biochemistry
  • 1979
The substrates phosphoenolpyruvate and erythrose 4-phosphate and the product inorganic phosphate can protect the purified enzyme against heat denaturation, whereas the inhibitor, tryptophan, has no effect, although it binds to the enzyme in the absence of other ligands.

Characterization of a recombinant type II 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Helicobacter pylori.

Analysis of type II DAH7P synthase sequences reveals several highly conserved motifs, and comparison with the type I enzymes suggests that catalysis by these two enzyme types occurs on a similar active-site scaffold and that the two DAH6P synthases families may indeed be distantly related.

Studies on 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase(phe)from Escherichia coli K12. 2. Kinetic properties.

The effects of pH ON V and V/(Km for phosphoenolpyruvate) indicated that an ionizing group of pKa 8.0-8.1 is involved in the catalytic activity of the enzyme and the pKa of this group is unaffected by the binding of phosphate.

Regulation of 3-deoxy-d-arabinoheptulosonic acid 7-phosphate synthetase in Nocardia mediterranei

As rifamycin and aromatic amino acid biosynthesis are closely interrelated, the regulation of DAHP-synthetase was investigated and the origin of this seven-carbon amino starter unit was located as being between 3-deoxy-D-arabinoheptulosonic acid-7-phosphate and shikimate.

Purification and Properties of 3-Deoxy-d-Arabinoheptulosonic Acid-7-Phosphate Synthetase (trp) from Escherichia coli

The 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthetase which is subject to regulation by tryptophan has been partially purified from a strain of Escherichia coli K-12, in which this is the

Properties of chorismate mutase ofStreptomyces venezuelae

Chorismate mutase, the first enzyme of the terminal biosynthetic pathway of phenylalanine and tyrosine has been purified 10 fold from Streptomyces venezuelae and the activity of the enzyme is inhibited by none of these substances.

Regulation of aromatic amino acid biosynthesis in Escherichia coli K-12: control of the aroF-tyrA operon in the absence of repression control

It is proposed that in the proximal region of the aroF-tyrA operon there is an attenuator site controlled by the level of charged tryptophanyl-transfer RNA that leads to an increased rate of synthesis of tyrosine-repressible DAHP synthetase during subsequent growth.

Autoregulation of the tyrR gene

The levels of beta-galactosidase synthesized in the tyrR::Mu d (Apr lac) strains indicate that tyrR has a relatively efficient promoter, the maximum levels representing on the order of a relatively effective promoter, and themaximum levels representing 1,000 monomers of Beta-galactsidase per cell in the TyrR strain and about 500 monomers inThe tyrR+ haploid strain.

References

SHOWING 1-10 OF 18 REFERENCES

Chromatography of 3-deoxy-D-arabinoheptulosonic acid-7-phosphate synthetase (trp) on diethylaminoethyl cellulose: a correction

The purpose of this note is to explain the nature of these artifacts and to present the elution profile of DAHP synthetase (trp), which is found in cell-free extracts prepared from cells grown in minimal medium alone and found a single peak of enzyme activity in fractions 37 to 40.

Genetic and Biochemical Analysis of the Isoenzymes Concerned in the First Reaction of Aromatic Biosynthesis in Escherichia coli

Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the

Estimation of the molecular weights of proteins by Sephadex gel-filtration.

  • P. Andrews
  • Biology, Computer Science
    The Biochemical journal
  • 1964
The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.