3-D observation of actin filaments during cardiac myofibrinogenesis in chick embryo using a confocal laser scanning microscope

@article{Shiraishi19923DOO,
  title={3-D observation of actin filaments during cardiac myofibrinogenesis in chick embryo using a confocal laser scanning microscope},
  author={Isao Shiraishi and T. Takamatsu and Tetsuhiro Minamikawa and Setsuya Fujita},
  journal={Anatomy and Embryology},
  year={1992},
  volume={185},
  pages={401-408}
}
Using a confocal laser scanning microscope (CLSM), we observed subcellular three-dimensional (3-D) arrangements of actin filaments stained with fluorescein-labeled phalloidin during myofibrinogenesis of chick embryonic heart (7- to 13-somite stages). Serial optical tomograms were obtained from whole-mounted heart tubes and reconstructed into stereoscopic images. Development of myofibrils in myocardial differentiation considerably differed in inner and outer myocardial cell layers. In the outer… CONTINUE READING

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