3 ß-Hydroxysteroid Oxidoreductase in Suspension Cultures of Digitalis lanata


A 3 ß-hydroxysteroid oxidoreductase was isolated and characterized in the microsomes o f Digitalis lanata cell cultures. The enzyme catalyzes the conversion o f 5a-pregnane-3,20-dione to 5a-pregnan-3 ß-ol-20-one and requires N A D (P)H 2. The enzyme was found to have a pH optimum o f 8.0. The reaction had an optimum incubation temperature o f 25 °C with… (More)