3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets

@article{Birmingham20063US,
  title={3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets},
  author={Amanda Birmingham and Emily M. Anderson and Angela Reynolds and Diane Ilsley-Tyree and Devin Leake and Yuriy Fedorov and Scott Baskerville and Elena M Maksimova and Kathryn Robinson and Jon M. Karpilow and William S. Marshall and Anastasia Khvorova},
  journal={Nature Methods},
  year={2006},
  volume={3},
  pages={199-204}
}
Off-target gene silencing can present a notable challenge in the interpretation of data from large-scale RNA interference (RNAi) screens. We performed a detailed analysis of off-targeted genes identified by expression profiling of human cells transfected with small interfering RNA (siRNA). Contrary to common assumption, analysis of the subsequent off-target gene database showed that overall identity makes little or no contribution to determining whether the expression of a particular gene will… 

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