3′ End cDNA amplification using classic RACE

  title={3′ End cDNA amplification using classic RACE},
  author={Elizabeth Scotto–Lavino and Guangwei Du and Michael A. Frohman},
  journal={Nature Protocols},
Having knowledge of the entire 3′ sequence of a cDNA is often important because the non-coding terminal region can contain signals that regulate the stability or subcellular localization of the mRNA. Also, some messages use alternative genomic sites for cleavage and polyadenylation that can alter the above properties, or change the encoded protein. Full-length cDNAs can be obtained from complex mixtures of cellular mRNA using rapid amplification of cDNA ends (RACE) PCR as long as part of the… CONTINUE READING

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