3'-phosphodiesterase activity of human apurinic/apyrimidinic endonuclease at DNA double-strand break ends.

@article{Suh19973phosphodiesteraseAO,
  title={3'-phosphodiesterase activity of human apurinic/apyrimidinic endonuclease at DNA double-strand break ends.},
  author={Dongchul Suh and David M Wilson and Lawrence F. Povirk},
  journal={Nucleic acids research},
  year={1997},
  volume={25 12},
  pages={2495-500}
}
In order to assess the possible role of human apurinic/apyrimidinic endonuclease (Ape) in double-strand break repair, the substrate specificity of this enzyme was investigated using short DNA duplexes and partial duplexes, each having a single 3'-phosphoglycolate terminus. Phosphoglycolate removal by Ape was detected as a shift in mobility of 5'-end-labeled DNA strands on polyacrylamide sequencing gels, and was quantified by phosphorimaging. Recombinant Ape efficiently removed phosphoglycolates… CONTINUE READING

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