2D differential in-gel electrophoresis for the identification of esophageal scans cell cancer-specific protein markers.

  title={2D differential in-gel electrophoresis for the identification of esophageal scans cell cancer-specific protein markers.},
  author={Ge Zhou and Hongmei Li and Dianne L Decamp and She Chen and Hongjun Shu and Yi Gong and M. Flaig and John Williams Gillespie and Nan Hu and Philip R. Taylor and Michael R. Emmert-Buck and Lance A. Liotta and Emanual F. Petricoin and Yingming Zhao},
  journal={Molecular & cellular proteomics : MCP},
  volume={1 2},
The reproducibility of conventional two-dimensional (2D) gel electrophoresis can be improved using differential in-gel electrophoresis (DIGE), a new emerging technology for proteomic analysis. In DIGE, two pools of proteins are labeled with 1-(5-carboxypentyl)-1'-propylindocarbocyanine halide (Cy3) N-hydroxy-succinimidyl ester and 1-(5-carboxypentyl)-1'-methylindodi-carbocyanine halide (Cy5) N-hydroxysuccinimidyl ester fluorescent dyes, respectively. The labeled proteins are mixed and separated… CONTINUE READING
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