27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells.

@article{Cruz201227hydroxycholesterolAT,
  title={27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells.},
  author={Pamela Cruz and Mar{\'i}a Jos{\'e} Epu{\~n}{\'a}n and Mar{\'i}a Eugenia Ram{\'i}rez and Cristian Gabriel Torres and Luis Emilio Valladares and Walter Daniel Sierralta},
  journal={Oncology reports},
  year={2012},
  volume={28 3},
  pages={992-8}
}
The principal aim of this study was to analyze in estrogen receptor-positive MCF7 cells the response of three estrogen-dependent proteins to 27-hydroxycholesterol (27OHC), a major circulating cholesterol metabolite. Immunofluorescence, immunoblotting and immunogold labelling analyses of MCF7 cells exposed for up to 72 h to 2 nM estradiol (E2) or to 2 µM 27OHC demonstrated similar responses in the expression of MnSOD and ERβ compared to… CONTINUE READING

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