25-Hydroxylation of vitamin D3 in the human hepatoma cell lines Hep G2 and Hep 3B.

@article{Tam198825HydroxylationOV,
  title={25-Hydroxylation of vitamin D3 in the human hepatoma cell lines Hep G2 and Hep 3B.},
  author={Shui Pang Tam and Stephen Strugnell and R. G. Deeley and Glenville Jones},
  journal={Journal of lipid research},
  year={1988},
  volume={29 12},
  pages={1637-42}
}
Two human hepatoma cell lines, Hep G2 and Hep 3B, were screened for vitamin D3-25-hydroxylase enzyme activity by incubation with radioactive vitamin D3. A compound co-chromatographing with 25-OH-D3 was synthesized in both cell lines but its rate of synthesis was tenfold greater in Hep 3B than in Hep G2 cells. The identity of the compound was confirmed by comparing its chromatographic properties with authentic 25-OH-D3 on three different high pressure liquid chromatography systems. Its… CONTINUE READING