24-Hydroxylation of 1,25-dihydroxyergocalciferol. An unambiguous deactivation process.

@article{Horst198624HydroxylationO1,
  title={24-Hydroxylation of 1,25-dihydroxyergocalciferol. An unambiguous deactivation process.},
  author={Ronald L. Horst and Timothy A Reinhardt and Charles F. Ramberg and Nicholas J. Koszewski and Joseph L. Napoli},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 20},
  pages={
          9250-6
        }
}
1,24,25-Trihydroxyergocalciferol was isolated from bovine kidney homogenates incubated with 1,25-dihydroxyergocalciferol and from chick kidney homogenates incubated with 24,25-dihydroxyergocalciferol. The identity was established by ultraviolet absorbance, sensitivity to periodate, nuclear magnetic resonance, and mass spectrometry. The new metabolite had an affinity equal to 1,24,25-trihydroxycholecalciferol for the bovine-thymus and chick-intestinal 1,25-dihydroxyvitamin D receptor and had an… Expand
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C-24 oxidation is a significant pathway of intestinal 1,25-dihydroxyvitamin D3 metabolism that produces metabolites with high affinity for the cytosolic receptor which mediates vitamin D action. Expand
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(23S)-23,25-Dihydroxycholecalciferol was converted into a polar metabolite in a calciferol-deficient chick kidney homogenate, and the trihydroxy metabolite was 50-fold less potent than 1, 25-dihydroxchole Calciferl in the chick intestinal 1,24-diethyltestosterone-treated rat receptor assay. Expand
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TLDR
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