23S rRNA similarity from selection for peptidyl transferase mimicry.

@article{Welch199723SRS,
  title={23S rRNA similarity from selection for peptidyl transferase mimicry.},
  author={Margaret R Welch and Irene H. Majerfeld and Michael Yarus},
  journal={Biochemistry},
  year={1997},
  volume={36 22},
  pages={
          6614-23
        }
}
RNAs from a randomized pool were selected by affinity elution for binding to the molecule CCdApPuro, a high-affinity ligand of ribosomal peptidyl transferase designed as a transition-state analogue of peptide formation. The selected RNAs show affinity for CCdApPuro comparable to that of the peptidyl transferase center itself (Kd approximately 10 nM). Chemical modification/protection experiments implicate bases completely conserved among the selected RNAs in CCdApPuro interaction, which appears… CONTINUE READING
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