225 Reserve Bilirubin Binding Capacity (rbbc) Measured by Absorbance Deviation (ad) at Saturation of Primary Albumin-binding Sites (pabs)


This method requires the sequential addition of 5 μl increments of bilirubin solution (BS) of 25 mg/dl to 50 μl of neonatal serum (NS) in 2 ml of 200 mM phosphate buffer, pH 7.4. With each addition of BS to the NS, there is a constant increase in absorbance at 484 nm until all PABS are exceeded. The table shows the expected absorbance change (Δ A484 nm) per… (More)
DOI: 10.1203/00006450-198104001-00234