22 genes from chromosome 17q21: cloning, sequencing, and characterization of mutations in breast cancer families and tumors.

@article{Friedman199522GF,
  title={22 genes from chromosome 17q21: cloning, sequencing, and characterization of mutations in breast cancer families and tumors.},
  author={Lawrence S Friedman and Elizabeth A. Ostermeyer and Eric D. Lynch and Piri I. Welcsh and Csffla I. Szabo and J E Meza and L A Anderson and Patrick Dowd and Ming Kai Lee and Sarah E. Rowell},
  journal={Genomics},
  year={1995},
  volume={25 1},
  pages={256-63}
}
In our effort to identify BRCA1, 22 genes were cloned from a 1-Mb region of chromosome 17q21 defined by meiotic recombinants in families with inherited breast and/or ovarian cancer. Subsequent discovery of another meiotic recombinant narrowed the region to approximately 650 kb. Genes were cloned from fibroblast and ovarian cDNA libraries by direct screening with YACs and cosmids. The more than 400 cDNA clones so identified were mapped to cosmids, YACs, and P1 clones and to a chromosome 17… CONTINUE READING