21 OLIGO Primer Analysis Software

@inproceedings{Offerman200321OP,
  title={21 OLIGO Primer Analysis Software},
  author={John D. Offerman and Wojciech Rychlik},
  year={2003}
}
Concurrent with the development of the polymerase chain reaction (PCR) in the late 1980s as an essential technique in the molecular biology laboratory, optimal design of PCR primers became an important part of the process. It was soon learned, for example, that inattention to secondary structure in synthetic primers could create primer-dimers and hairpins and could seriously reduce or eliminate the yield of the PCR product. The presence of priming sites in the template other than the intended… CONTINUE READING

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