• Corpus ID: 55812

20 Years of Transgenic Research in China for Resistance to Papaya ringspot virus

@inproceedings{Ye201020YO,
  title={20 Years of Transgenic Research in China for Resistance to Papaya ringspot virus},
  author={Changming Ye and Huaping Li},
  year={2010}
}
Papaya is a favorite fruit and important industrial raw material in China. It is traditionally planted in southern China. Due to its wide uses and mystical health-related effects, papaya has been listed in fruits of high priority. Papaya ringspot virus (PRSV) is the main limitation of papaya production. PRSV infection rate reaches 100% in field in the late season of planting, which considerably reduces productivity and quality of papaya. Several research groups in China started around 1990 to… 

Figures and Tables from this paper

Characterization of Papaya ringspot virus isolates infecting transgenic papaya ‘Huanong No.1’ in South China
TLDR
The results indicate that the genetic variation of PRSV and the formation of the new virus lineage may explain the loss of transgenic papaya resistance in South China.
Transgene-mediated resistance to Papaya ringspot virus: challenges and solutions
TLDR
The focus is on factors affecting transgenic resistance in papaya and the attempt to explain why the Hawaiian scenario of complete and durable resistance has not been replicated in other regions.
Variation of Neomycin Phosphotransferase II Marker Gene in Transgenic Papaya Plants Under the Field Cultivation
TLDR
The results indicate that selective marker genes in transgenic plants can be naturally deleted by mutation and that marker-free transgenic Plants can be obtained for basal plant materials to develop desirable transgenic crops for commercialization.
Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Papaya ringspot virus.
TLDR
It is suggested that PRSV remains the major limiting factor for non-transgenic-papaya production, and the emergence of PLDMV threatens the commercial transgenic cultivar in China.
Genetically Modified Crops: Current Status, Prospects and Challenges Volume 2
Tomato (Lycopersicon esculentum Mill.) is the second most important vegetable crop of the world. It is rich in nutrition with zero cholesterol, but highly sensitive to abiotic stresses, especially
Engineering Plant Virus Resistance: Gene Silencing to Genome Editing
TLDR
The rate of grain production does not seem to have kept pace with population growth across the globe, and the global food production is also limited by insect pests and disease causing pathogens.
Resistance of non-transgenic papaya plants to papaya ringspot virus (PRSV) mediated by intron-containing hairpin dsRNAs expressed in bacteria.
TLDR
It is indicated that direct mechanical inoculation of papaya plants with bacterially-expressed ihpRNA-CP279 targeting the PRSV CP gene can interfere with virus infection.
Simultaneous detection of papaya ringspot virus, papaya leaf distortion mosaic virus, and papaya mosaic virus by multiplex real-time reverse transcription PCR.
TLDR
This multiplex real-time RT-PCR assay provides a more rapid, sensitive and reliable method for simultaneous detection of PRSV, PLDMV, PapMV and their mixed infections in papaya.
Transgenic and genome-edited fruits: background, constraints, benefits, and commercial opportunities
TLDR
This review, emphasizes the socioeconomic impact of current commercial fruit cultivars developed by genetic engineering and the potential impact of genome editing on the development of improved cultivars at an accelerated rate.
Engineering plant virus resistance: from RNA silencing to genome editing strategies
Summary Viral diseases severely affect crop yield and quality, thereby threatening global food security. Genetic improvement of plant virus resistance is essential for sustainable agriculture. In the
...
1
2
...

References

SHOWING 1-10 OF 52 REFERENCES
Analyses of virus resistance and fruit quality for T4 generation of transgenic papaya
TLDR
All transgenic papaya plants with the mutated replicase(RP) gene from papaya ringspot virus showed highly resistant or immune against PRV in field, and there was no significant difference between transgenic and non-transgenic plants.
Transgenic papaya: development, release, impact and challenges.
  • D. Gonsalves
  • Biology, Medicine
    Advances in virus research
  • 2006
TLDR
This chapter considers the development, testing, release and impact of the virus-resistant transgenic papaya in Hawaii, and the efforts to transfer the technology to Jamaica, Venezuela, and Thailand.
Evaluation of resistance to PRSV and selection of homogeneous lineon the transgenic papaya plants
TLDR
All plants of Trp-6-2, no matter its self- pollinated or cross-pollinated with any susceptible cultivars, showed that no any symptom appeared after inoculation in the green house, and Trp gene could be detected, indicating that Trp might be one of homogenous transgenic line.
Evaluation of PRSV resistance of T_2 transgenic papaya with replicase gene
Results from PCR and southern-blot analysis conformed that the target gene was integrated into the genome of T_2 transgenic papaya (Carica papaya) plants with replicase gene of papaya ringspot virus
The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus)
TLDR
Papaya offers numerous advantages as a system for fruit-tree functional genomics, and this draft genome sequence provides the foundation for revealing the basis of Carica’s distinguishing morpho-physiological, medicinal and nutritional properties.
Virus-Induced Gene Silencing-Mediated Viral Resistance of Transgenic Papaya to Papaya ringspot virus(PRSV)
TLDR
The resistance of transgenic papaya plants was caused by virus-induced gene silencing, and an relation between transgene transcript accumulation and virus resistance was revealed.
Preliminary Research of PRSV-CP Gene Transferred into Papaya by Pollen-tube Pathway
With Papaya line of solo Ⅱ as receptor,278 bp fragment of Papaya ringspot virus coat protein (PRSV-CP)gene was transferred by pollen-tube pathway,which flowers were dealed with plasmid DNA (187 of
Field Released Transgenic Papaya Affects Microbial Communities and Enzyme Activities in Soil
TLDR
It is suggested that transgenic papaya could alter chemical properties, enzyme activities, and microbial communities in soil.
An Initial Study of Transgenic Carica papaya Used as a Kind of Vaccine for Anti-Tuberculosis
TLDR
These transgenic plants will provide new m aterial for the development of edible vaccines for anti- tuberculosis using ESAT-6 gene, which was modified by PC R and integrated into a binary vector pBI121.
Study on the compositional differences between transgenic and non-transgenic papaya (Carica papaya L.)
TLDR
It is hoped that this study could provide some reference value for a safety evaluation of transgenic papaya from the compositional point of view, and could also propose a method for discrimination oftransgenic food from non-transgenic counterparts.
...
1
2
3
4
5
...