20 S proteasomes are imported as precursor complexes into the nucleus of yeast.

@article{Lehmann200220SP,
  title={20 S proteasomes are imported as precursor complexes into the nucleus of yeast.},
  author={Andrea Lehmann and Katharina Janek and Beate Braun and Peter M Kloetzel and Cordula Enenkel},
  journal={Journal of molecular biology},
  year={2002},
  volume={317 3},
  pages={401-13}
}
The mechanism by which yeast 20 S proteasomes are imported into the nucleus is still unresolved. Here, we provide the first evidence that 20 S proteasomes are imported as precursor complexes into the nucleus. By using the srp1-49 mutant which is deficient in nuclear import of cargos with classical nuclear localization sequences (cNLS), we show that proteasome precursor complexes associate with importin/karyopherin alphabeta, the cNLS receptor, and that they accumulate inside the cytoplasm… CONTINUE READING