2.0 Å resolution crystal structure of human polκ reveals a new catalytic function of N-clasp in DNA replication

@inproceedings{Jha201820R,
  title={2.0 {\AA} resolution crystal structure of human polκ reveals a new catalytic function of N-clasp in DNA replication},
  author={Vikash Jha and Hong Ling},
  booktitle={Scientific Reports},
  year={2018}
}
Human polymerase kappa (polκ) is a distinct Y-family DNA polymerase with a unique N-terminal N-clasp domain. The N-clasp renders polκ’s high efficiency and accuracy in DNA replication and lesion bypass. How N-clasp empowers polκ in replication remains unclear due to the disordering of N-clasp. Here, we present a 2.0-Å resolution crystal structure of a polκ ternary complex with DNA and an incoming nucleotide. The structure-function study reveals an ordered N-clasp domain that brings conserved… CONTINUE READING
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