2-Naphthoate catabolic pathway in Burkholderia strain JT 1500

  title={2-Naphthoate catabolic pathway in Burkholderia strain JT 1500},
  author={B Morawski and Richard W. Eaton and John Trevor Rossiter and Sun Guoping and Herfried Griengl and Douglas W. Ribbons},
  journal={Journal of Bacteriology},
  pages={115 - 121}
Burkholderia strain (JT 1500), able to use 2-naphthoate as the sole source of carbon, was isolated from soil. On the basis of growth characteristics, oxygen uptake experiments, enzyme assays, and detection of intermediates, a degradation pathway of 2-naphthoate is proposed. The features of this pathway are convergent with those for phenanthrene. We propose a pathway for the conversion of 2-naphthoate to 1 mol (each) of pyruvate, succinate, and acetyl coenzyme A and 2 mol of CO2. During growth… Expand
Characterization of two components of the 2-naphthoate monooxygenase system from Burkholderia sp. strain JT1500.
Data suggest that NmoB(T)A is a two-component flavoprotein monooxygenase, consisting of an oxygenase and a reductase component, and NmoA is an independent NADH:Flavin oxidoreductase. Expand
Oxidation of Methyl-Substituted Naphthalenes: Pathways in a Versatile Sphingomonas paucimobilisStrain
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Quantification of substrate consumption, sulphide formation and formed cell mass revealed that naphthalene was completely oxidized with sulphate as the electron acceptor. Expand
Degradation of 2-Methylnaphthalene by a Sulfate-Reducing Enrichment Culture of Mesophilic Freshwater Bacteria
Granules of expanded clay ("Lecaton") serving as a mineral filter bed in a groundwater purification plant (Lübeck, Metallhüttengelände) were investigated for the presence of mesophilic freshwaterExpand
Proteomic and transcriptional characterization of aromatic degradation pathways in Rhodoccocus sp. strain TFB
Absence of catabolite repression by glucose was observed by both transcriptional and proteome analysis, suggesting that Rhodococcus sp. Expand
Biochemical and Genetic Characterization oftrans-2′-Carboxybenzalpyruvate Hydratase-Aldolase from a Phenanthrene-Degrading Nocardioides Strain
ABSTRACT trans-2′-Carboxybenzalpyruvate hydratase-aldolase was purified from a phenanthrene-degrading bacterium,Nocardioides sp. strain KP7, and characterized. The purified enzyme was found to haveExpand
In situ proton NMR analysis of alpha-alkynoate biotransformations. From 'invisible' substrates to detectable metabolites.
This work isolated a Pseudomonas putida strain growing on 2-butynedioate as well as on propynoate, and determined the metabolic pathways of these two alpha-alkynoates, finding that they are good substrates for the enzymes involved in metabolism of acetylenic compounds, resulting in products that are suitable for bacterial growth. Expand
Bioconversion of AHX to AOH by resting cells of Burkholderia contaminans CH-1
A bacterial strain CH-1 was isolated from an airborne-contaminated nutrient medium containing AHX and identified as Burkholderia contaminans based on the gene sequence of its 16S rDNA and the quantitative production of AOH by resting cells of the strain was achieved. Expand
Proton-Nuclear Magnetic Resonance Analyses of the Substrate Specificity of a β-Ketolase from Pseudomonas putida, Acetopyruvate Hydrolase
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Phthalate biodegradation: gene organization, regulation and detection
Sequence analyses indicate that phthalate, isophthalate and terephthalate degrading bacterial isolates at the same location are not simply clones of each other and that the genes identified are linked specifically to these bacterial strains. Expand


Degradation of Phenanthrene through o-Phthalate by an Aeromonas sp.
A phenanthrene-assimilating bacterium which belongs to the genus Aeromonas was isolated from soil. The cells which adapted to phenanthrene required a growth lag time on a naphthalene medium. TheExpand
Phthalate pathway of phenanthrene metabolism: formation of 2'-carboxybenzalpyruvate
The metabolism of phenanthrene by a gram-negative organism able to use this compound as a sole source of carbon and energy has been examined and the stoichiometry and UV spectrum were consistent with the identification of the product as 2'-carboxybenzalpyruvate. Expand
The Catabolism of Phenanthrene and Naphthalene by Bacteria
SUMMARY: Thirteen strains of bacteria able to grow on phenanthrene were isolated from soil; they included fluorescent and non-fluorescent pseudomonads, vibrios and unidentified bacteria. Two of theExpand
Microbial oxidation of dimethylnaphthalene isomers
Three bacterial strains, identified as Alcaligenes sp. strain D-59 and Pseudomonas sp. strains D-87 and D-186, capable of growing on 2,6-dimethylnaphthalene (2,6-DMN) as the sole source of carbon andExpand
Bacterial metabolism of naphthalene: construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxynaphthalene and subsequent reactions
The reactions involved in the bacterial metabolism of naphthalene to salicylate have been reinvestigated by using recombinant bacteria carrying genes cloned from plasmid NAH7, and the gene order for the nah operon was shown to be p, A, B, F, C, E, D. Expand
The Fe(2+)-dependent oxygenase responsible for cleavage of all the o-dihydroxyphenol derivatives appears to be catechol 2,3-oxygenase, and is a constitutive enzyme in the Pseudomonas strains used. Expand
Catabolism of Naphthalenesulfonic Acids by Pseudomonas sp. A3 and Pseudomonas sp. C22
Inhibition kinetics show that naphthalene and substituted naphthaenes are hydroxylated by the same nAPHthalene dioxygenase, which results in 1,2-dihydroxy-1, 2- dihydronaphthalenes-2-carboxylic acids are formed quantitatively from the corresponding n aphthalenecarboxYlic acids. Expand
Metabolism of 2,6-dimethylnaphthalene by flavobacteria
  • E. A. Barnsley
  • Chemistry, Medicine
  • Applied and environmental microbiology
  • 1988
Flavobacteria isolated from soil were able to oxidize a broad range of aromatic hydrocarbons after growth on 2,6-DMN at rates comparable to that of the oxidation of 2,4-dimethylnaphthalene itself. Expand
The Conversion of Catechol and Protocatechuate to β-Ketoadipate by Pseudomonas putida I. BIOCHEMISTRY
Abstract Two new intermediates were identified in the protocatechuate pathway of Pseudomonas putida. The first of these, γ-carboxymuconolactone (γ-carboxy-γ-carboxymethyl-Δα-butenolide), is theExpand
Two-Stage Mineralization of Phenanthrene by Estuarine Enrichment Cultures
The polycyclic aromatic hydrocarbon phenanthrene was mineralized in two stages by soil, estuarine water, and sediment microbial populations. At high concentrations, phenanthrene was degraded, withExpand