2-DE-based proteomic analysis of common bean (Phaseolus vulgaris L.) seeds.

  title={2-DE-based proteomic analysis of common bean (Phaseolus vulgaris L.) seeds.},
  author={M. C. de la Fuente and A. Borrajo and J. Bermudez and M. Lores and J. Alonso and M. Lopez and M. Santalla and A. D. De Ron and C. Zapata and G. {\'A}lvarez},
  journal={Journal of proteomics},
  volume={74 2},
  • M. C. de la Fuente, A. Borrajo, +7 authors G. Álvarez
  • Published 2011
  • Biology, Medicine
  • Journal of proteomics
  • Common bean (Phaseolus vulgaris L.) is the most important grain legume for direct human consumption. Proteomic studies in legumes have increased significantly in the last years but few studies have been performed to date in P. vulgaris. We report here a proteomic analysis of bean seeds by two-dimensional electrophoresis (2-DE). Three different protein extraction methods (TCA-acetone, phenol and the commercial clean-up kit) were used taking into account that the extractome can have a determinant… CONTINUE READING
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