2'-O,4'-C-ethylene bridged nucleic acid modification enhances pyrimidine motif triplex-forming ability under physiological condition.

@article{Torigoe20122O4CethyleneBN,
  title={2'-O,4'-C-ethylene bridged nucleic acid modification enhances pyrimidine motif triplex-forming ability under physiological condition.},
  author={Hidetaka Torigoe and Norihiro Sato and Nobuyuki Nagasawa},
  journal={Journal of biochemistry},
  year={2012},
  volume={152 1},
  pages={
          17-26
        }
}
Since pyrimidine motif triplex DNA is unstable at physiological neutral pH, triplex stabilization at physiological neutral pH is important for improvement of its potential to be applied to various methods in vivo, such as repression of gene expression, mapping of genomic DNA and gene-targeted mutagenesis. For this purpose, we studied the thermodynamic and kinetic effects of a chemical modification, 2'-O,4'-C-ethylene bridged nucleic acid (ENA) modification of triplex-forming oligonucleotide… CONTINUE READING
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