2'-Deoxy-2'-azidoadenosine triphosphate and 2'-deoxy-2'-fluoroadenosine triphosphate as substrates and inhibitors for Escherichia coli DNA-dependent RNA polymerase.

@article{Ishihama19802Deoxy2azidoadenosineTA,
  title={2'-Deoxy-2'-azidoadenosine triphosphate and 2'-deoxy-2'-fluoroadenosine triphosphate as substrates and inhibitors for Escherichia coli DNA-dependent RNA polymerase.},
  author={A. Ishihama and M. Enami and Y. Nishijima and T. Fukui and E. Ohtsuka and M. Ikehara},
  journal={Journal of biochemistry},
  year={1980},
  volume={87 3},
  pages={
          825-30
        }
}
  • A. Ishihama, M. Enami, +3 authors M. Ikehara
  • Published 1980
  • Biology, Medicine
  • Journal of biochemistry
  • The effects of 2'-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the presence of UTP, 2'-deoxy-2'-azidoadenosine 5'-triphosphate (AZTP) was incorporated into an acid-insoluble fraction at one-tenth of the rate of ATP incorporation; it thus acts as a competitive inhibitor for poly(AU) synthesis. On the other hand, another ATP analog, 2'-deoxy-2'-fluoroadenosine 5… CONTINUE READING
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