2‐Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter

@article{VazquezBermudez20022OxoglutarateIT,
  title={2‐Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter},
  author={M. F. Vázquez-Bermúdez and A. Herrero and E. Flores},
  journal={FEBS Letters},
  year={2002},
  volume={512}
}
The cyanobacterial NtcA global nitrogen regulator belongs to the catabolite activator protein (CAP) family and activates transcription of nitrogen assimilation genes in response to nitrogen step‐down. The binding affinity of NtcA towards a DNA fragment carrying the promoter of the glnA gene from Synechococcus sp. PCC 7942, analyzed in vitro by band‐shift assay, was increased five‐fold by 2‐oxoglutarate in the presence of Mg2+ ions. The 2‐oxoglutarate effect peaked at about 0.6 mM, a rather… Expand
Transcription Activation by NtcA and 2-Oxoglutarate of Three Genes Involved in Heterocyst Differentiation in the Cyanobacterium Anabaena sp. Strain PCC 7120
TLDR
A stringent requirement of both NtcA and 2-Oxoglutarate was observed for the detection of open complexes and transcript production at the three investigated promoters, which support a key role for 2-oxoglUTarate in transcription activation in the developing heterocyst. Expand
In vivo activity of the nitrogen control transcription factor NtcA is subjected to metabolic regulation in Synechococcus sp. strain PCC 7942.
TLDR
Results indicate in vivo regulation of the activity of NtcA at activation of transcription of nitrogen-regulated genes as a function of the nitrogen status of the cell. Expand
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TLDR
The results suggest that the 2‐oxoglutarate level, as a signal of the C to N balance of the cells, regulates the microcystin gene cluster. Expand
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TLDR
Results indicate that PipX interacts with NtcA to facilitate efficient acclimation of cyanobacteria to conditions of nitrogen limitation, and the effect of pipX inactivation on various nitrogen-regulated processes was determined. Expand
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A trend to streamline the regulation of C/N metabolism in late-branching Prochlorococcus strains (MED4 and SS120), in adaptation to the rather stable conditions found in the oligotrophic ocean gyres where this microorganism is most abundant is suggested. Expand
Transcriptional effects of the signal transduction protein PII (glnB gene product) on NtcA‐dependent genes in Synechococcus sp. PCC 7942
TLDR
The analysis of gene expression through RNA/DNA hybridization indicated that a PII‐null mutant was also impaired in the induction of NtcA‐dependent, nitrogen assimilation genes amt1 (ammonium permease), glnA (glutamine synthetase) and nir (nitrite reductase), as well as of the N‐control gene ntcA, mainly under nitrogen deprivation. Expand
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Expression of the glutamyl‐tRNA synthetase gene from the cyanobacterium Synechococcus sp. PCC 7942 depends on nitrogen availability and the global regulator NtcA
We report here transcriptional analyses of a cyanobacterial gene encoding an aminoacyl‐tRNA synthetase (aaRS), the gltX gene from Synechoccocus sp. PCC 7942, coding for the glutamyl‐tRNA synthetase.Expand
Carbon supply and 2-oxoglutarate effects on expression of nitrate reductase and nitrogen-regulated genes in Synechococcus sp. strain PCC 7942.
TLDR
Results are consistent with a role of 2-oxoglutarate in nitrogen control in cyanobacteria and at the mRNA level for two nitrogen-regulated genes, nir and amt1, but not for the photosystem II D1 protein-encoding gene psbA1. Expand
Analysis of a non-canonical NtcA-dependent promoter in Synechococcus elongatus and its regulation by NtcA and PII
TLDR
A genetic analysis of the glnN promoter region of Synechococcus elongatus PCC 7942 revealed a non-canonical NTCA-binding motif is responsible for NtcA-dependent, nitrogen-responsive regulation of gln n gene expression and sequences upstream of the Ntc a-binding site have repressive effect on gln N promoter activity. Expand
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