2,5-Dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn derivatization reagent for HPLC/UV detection of amino acids.

@article{Gatti201025Dimethyl1Hpyrrole34dicarbaldehydeA,
  title={2,5-Dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn derivatization reagent for HPLC/UV detection of amino acids.},
  author={Rita Gatti and Maria Grazia Gioia and Alberto Leoni and Aldo Andreani},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2010},
  volume={53 2},
  pages={207-11}
}
The use of 2,5-dimethyl-1H-pyrrole-3,4-dicarbaldehyde as a precolumn derivatization reagent for HPLC analysis of amino acids is proposed. The compound reacts under mild conditions (10min at ambient temperature) with primary amino groups. The derivatization conditions to obtain quantitative reaction were optimised by considering different parameters (temperature, pH and reagent concentration) using l-Val as the model compound. The synthesized l-Val derivative was characterized by (1)H NMR and UV… CONTINUE READING