2,4-Dinitrophenol does not reduce ATP levels in starving hamster fibroblasts although hexose transport regulation is markedly affected

  title={2,4-Dinitrophenol does not reduce ATP levels in starving hamster fibroblasts although hexose transport regulation is markedly affected},
  author={Eliezer Rapaport and Paul Plesner and Donna B. Ullrey and Herman M. Kalckar},
  journal={Carlsberg Research Communications},
In glucose-fed hamster fibroblast cultures maintained in a serum- and glutamine-free medium, DNP (2,4-dinitrophenol) has no effect on the total cellular pool of ATP. Under these circumstances a marked effect of DNP on the hexose transport system can be demonstrated. 
4 Citations
Studies of Regulation of Hexose Transport into Cultured Fibroblasts
The main theme will be the effects of carbohydrates and their metabolism in relation to the regulation of uptake or transport of hexoses into cultured fibroblasts, including special metabolic mutants.
Hexokinase Binding to Mitochondria: A Basis for Proliferative Energy Metabolism
Evidence is presented suggesting that the increased binding of Hexokinase to porin on mitochondria of cancer cells not only accelerates glycolysis by providing hexokinase with better access to ATP, but also stimulates the TCA cycle by providing the mitochondrion with ADP that acts as an acceptor for phosphoryl groups.


Uncouplers of oxidative phosphorylation promote derepression of the hexose transport system in cultures of hamster cells.
DNP, and other uncouplers of oxidative phosphorylation, promoted a derepressed state of hexose transport but did not stimulate the generation of lactate from glucosamine, suggesting that the metabolic repression phenomena ofhexose transport do not depend on glycolysis but rather on the "energized" state of the cell.
Effects of combined glutamine and serum deprivation on glucose control of hexose transport in mammalian fibroblast cultures.
Regulation of hexose transport in NIL hamster fibroblasts has been studied in confluent cultures preconditioned for 24 hr in media deprived of glutamine or of serum or of both and the various phenomena are discussed.
Derepression and carrier turnover: Evidence for two distinct mechanisms of hexose transport regulation in animal cells
The results indicate that the turnover mechanism becomes inactive during glucose starvation and must be resynthesized following refeeding of the starved cells with glucose.
Selective high metabolic lability of uridine, guanosine and cytosine triphosphates in response to glucose deprivation and refeeding of untransformed and polyoma virus‐transformed hamster fibroblasts
Measurements of hexose uptake by NIL and PyNIL cells under a variety of conditions suggest that hexose transport is not regulated by the total cellular pools of ATP or any of the other ribonucleoside triphosphates.
Two distinct types of enhancement of galactose uptake into hamster cells: tumor-virus transformation and hexose starvation.
  • H. Kalckar, D. Ullrey
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1973
Enhancement of hexose uptake seems well correlated with transformation of cell cultures by tumor viruses and the absence of contact inhibition. Enhancement of sugar uptake has also been observed as a
Protein measurement with the Folin phenol reagent.
Extraction procedures for use prior to HPLC nucleotide analysis using microparticle chemically bonded packings.
Tri-octyl-amine-freon solutions can be used for the extraction of the acid from the solution if the concentration of the amine is carefully controlled.
Long-term regulation of amino acid and hexose transport in cultured animal cells.