1H-NMR studies on nucleotide binding to the catalytic sites of bovine mitochondrial F1-ATPase.

Abstract

The conformation of adenine nucleotides bound to bovine mitochondrial F1-ATPase was investigated using transfer nuclear Overhauser enhancement measurements. It is shown that all nucleotides investigated adopt a predominantly anti conformation when bound to the catalytic sites. Furthermore, the experiment suggests that 8-azido-ADP and 8-azido-ATP, which are predominantly in the syn conformation in solution, are in the anti conformation when bound to F1 catalytic sites.

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Cite this paper

@article{Garin19881HNMRSO, title={1H-NMR studies on nucleotide binding to the catalytic sites of bovine mitochondrial F1-ATPase.}, author={J{\'e}r{\^o}me Garin and Pierre V. Vignais and Angela M. Gronenborn and G Marius Clore and Zengqiang Gao and Edmund B{\"a}uerlein}, journal={FEBS letters}, year={1988}, volume={242 1}, pages={178-82} }