1H-NMR detectable fatty acyl chain unsaturation in excised leiomyosarcoma correlate with grade and mitotic activity.

@article{Singer19961HNMRDF,
  title={1H-NMR detectable fatty acyl chain unsaturation in excised leiomyosarcoma correlate with grade and mitotic activity.},
  author={Sara J. Singer and M Sivaraja and Kenneth Souza and Kevin K. Millis and Joseph M. Corson},
  journal={The Journal of clinical investigation},
  year={1996},
  volume={98 2},
  pages={244-50}
}
We report on the use of 1H-NMR two-dimensional total correlated spectroscopy (2D TOCSY) at 600 MHz for an ex vivo analysis of fatty acyl chain lipid in normal smooth muscle and a series of primary retroperitoneal leiomyosarcomas. These TOCSY spectra were used to identify and quantitate the methylene protons situated between unsaturated site protons (D) to those bordered by only one unsaturated site proton (C). The D/C cross-peak volume ratios determined for oleic (18:1), linoleic (18:2… CONTINUE READING