17alpha-ethynylestradiol-induced vitellogenin gene transcription quantified in livers of adult males, larvae, and gills of fathead minnows (Pimephales promelas).

@article{Lattier200217alphaethynylestradiolinducedVG,
  title={17alpha-ethynylestradiol-induced vitellogenin gene transcription quantified in livers of adult males, larvae, and gills of fathead minnows (Pimephales promelas).},
  author={David L Lattier and T. Vijaya Bhaskara Reddy and Denise A Gordon and James M. Lazorchak and M. Elliot Smith and Daniel E. Williams and Barry Wiechman and Robert W Flick and Ann L Miracle and Gregory P Toth},
  journal={Environmental toxicology and chemistry},
  year={2002},
  volume={21 11},
  pages={2385-93}
}
We have applied a method for quantifying relative levels of messenger RNA (mRNA) transcription to assess chemically induced gene expression in fathead minnows (Pimephales promelas). Synthetic oligonucleotides designed for the fathead minnow vitellogenin gene transcription product were used in a reverse transcription polymerase chain reaction (RT-PCR… CONTINUE READING