Corpus ID: 25146301

17alpha-ethynylestradiol-induced vitellogenin gene transcription quantified in livers of adult males, larvae, and gills of fathead minnows (Pimephales promelas).

@article{Lattier200217alphaethynylestradiolinducedVG,
  title={17alpha-ethynylestradiol-induced vitellogenin gene transcription quantified in livers of adult males, larvae, and gills of fathead minnows (Pimephales promelas).},
  author={D. Lattier and T. Reddy and D. Gordon and J. Lazorchak and Mark E. Smith and D. Williams and B. Wiechman and R. Flick and A. Miracle and G. Toth},
  journal={Environmental toxicology and chemistry},
  year={2002},
  volume={21 11},
  pages={
          2385-93
        }
}
We have applied a method for quantifying relative levels of messenger RNA (mRNA) transcription to assess chemically induced gene expression in fathead minnows (Pimephales promelas). Synthetic oligonucleotides designed for the fathead minnow vitellogenin gene transcription product were used in a reverse transcription polymerase chain reaction (RT-PCR) protocol. This sensitive and rapid strategy detected vitellogenin gene transcription in livers of male fathead minnows exposed to concentrations… Expand
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