Corpus ID: 25146301

17alpha-ethynylestradiol-induced vitellogenin gene transcription quantified in livers of adult males, larvae, and gills of fathead minnows (Pimephales promelas).

  title={17alpha-ethynylestradiol-induced vitellogenin gene transcription quantified in livers of adult males, larvae, and gills of fathead minnows (Pimephales promelas).},
  author={D. Lattier and T. Reddy and D. Gordon and J. Lazorchak and Mark E. Smith and D. Williams and B. Wiechman and R. Flick and A. Miracle and G. Toth},
  journal={Environmental toxicology and chemistry},
  volume={21 11},
We have applied a method for quantifying relative levels of messenger RNA (mRNA) transcription to assess chemically induced gene expression in fathead minnows (Pimephales promelas). Synthetic oligonucleotides designed for the fathead minnow vitellogenin gene transcription product were used in a reverse transcription polymerase chain reaction (RT-PCR) protocol. This sensitive and rapid strategy detected vitellogenin gene transcription in livers of male fathead minnows exposed to concentrations… Expand
Characterization of ontogenetic changes in gene expression in the fathead minnow (Pimephales promelas).
Knowing of the normal changes in gene expression during embryo and larval development will allow for better experimental design and selection of suitable biomarkers when testing the potential toxicological effects of contaminants in this model fish species. Expand
Gene expression profiles in fathead minnow exposed to 2,4-DNT: correlation with toxicity in mammals.
  • H. Wintz, L. Yoo, +7 authors C. Vulpe
  • Biology, Medicine
  • Toxicological sciences : an official journal of the Society of Toxicology
  • 2006
It is suggested that fathead minnow cDNA microarrays can be useful to identify mechanisms of toxicity in fish and as a predictive tool for toxicity in mammals and possible modes of action of 2,4-DNT toxicity. Expand
Pulp and paper mill effluents induce distinct gene expression changes linked to androgenic and estrogenic responses in the fathead minnow (Pimephales promelas).
It is suggested that kraft effluent before and after biological treatment contained compounds able to induce androgenic effects in FHMs, and that combination of kraft and newsmill effluents eliminated the androgensic compounds while inducing distinct and significant patterns of gene expression changes that were likely due to estrogenic compounds produced by the newsmill. Expand
A computational model of the hypothalamic-pituitary-gonadal axis in male fathead minnows exposed to 17alpha-ethinylestradiol and 17beta-estradiol.
A physiologically based computational model was developed of the hypothalamic-pituitary-gonadal (HPG) axis that provides a robust representation of the HPG axis in male FHM and found good agreement between the model predictions and a variety of measured reproductive endpoints. Expand
Estrogenic potential of benzotriazole on marine medaka (Oryzias melastigma).
The results of the present study suggest that even at environmentally relevant concentrations detected in the aquatic environment, 0.01 mg/L, benzotriazole also caused notable changes in expression levels of VTG, CYP1A1 and CYP19a, and more concerns about the toxicity of benzotRIazoles on marine animals should be raised. Expand
Metformin Exposure at Environmentally Relevant Concentrations Causes Potential Endocrine Disruption in Adult Male Fish
The present study, the first to study the effects of environmentally relevant metformin exposure in fathead minnows, demonstrates the need for further study of the endocrine-disrupting effects of meetformin in aquatic organisms. Expand
Effects of Endocrine Disrupters on Sexual, Gonadal Development in Fish
In this review, an overview about field data is given and experimental evidence that links disruption of gonadal development in gonochoristic fish to contaminations by endocrine disrupting chemicals is summarized and categorised. Expand
Molecular biomarkers of endocrine disruption in small model fish
It is demonstrated that the available data clearly indicates the predictive potential of molecular biomarkers, supporting the development and regulatory application of simple molecular-based screening assays using small model fish for EDC testing. Expand
Environmental risk assessment and human pharmaceuticals : limitations and future improvements
A cocktail of human pharmaceuticals contaminate surface waters worldwide in the ng-?gl-1 range. Adverse effects on non target organisms including endocrine disruption and alterations in behaviour andExpand