16S ribosomal DNA-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by PCR and denaturing gradient gel electrophoresis.

@article{Kawai200216SRD,
  title={16S ribosomal DNA-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by PCR and denaturing gradient gel electrophoresis.},
  author={Mako Kawai and Eiichi Matsutera and Hisashi Kanda and Nobuyasu Yamaguchi and Katsuji Tani and Masao Nasu},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 2},
  pages={699-704}
}
The bacterial community in partially purified water, which is prepared by ion exchange from tap water and is used in pharmaceutical manufacturing processes, was analyzed by denaturing gradient gel electrophoresis (DGGE). 16S ribosomal DNA fragments, including V6, -7, and -8 regions, were amplified with universal primers and analyzed by DGGE. The bacterial diversity in purified water determined by PCR-DGGE banding patterns was significantly lower than that of other aquatic environments. The… CONTINUE READING

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