16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform

@inproceedings{Fouhy201616SRG,
  title={16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform},
  author={Fiona Fouhy and Adam G. Clooney and Catherine Stanton and Marcus J. Claesson and Paul D Cotter},
  booktitle={BMC Microbiology},
  year={2016}
}
Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer sequences and sequencing platform employed, can affect the accuracy of the results achieved. The aim of this study was to determine the impact of these three factors on 16S rRNA gene sequencing results, using mock… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 79 times over the past 90 days. VIEW TWEETS
31 Citations
31 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 31 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 31 references

Similar Papers

Loading similar papers…