16S rDNA-PCR and Sequencing Improves Diagnosis of Bacterial Infection of the Central Nervous System

  title={16S rDNA-PCR and Sequencing Improves Diagnosis of Bacterial Infection of the Central Nervous System},
  author={K. Boden and S. Sachse and M. Baier and K. Schmidt and M. Brodhun and R. Husain and E. Straube and S. Isenmann},
  journal={The Open Critical Care Medicine Journal},
Rapid initiation of antibiotic treatment and fast diagnosis are essential in bacterial infection of the central nervous system (CNS). Culture as common method for detecting bacteria is time consuming and unreliable once antibiotic treatment has been initiated. Eubacterial 16S rDNA-PCR with species differentiation by sequencing appears to be a promising tool. Our experiences with this method performed on specimens from patients with neurological disorders between 2004 and 2006 are presented. The… Expand
4 Citations


Aetiological diagnosis of brain abscesses and spinal infections: application of broad range bacterial polymerase chain reaction analysis
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