16 S rDNA primers and the unbiased assessment of thermophile diversity.

@article{Baker200416SR,
  title={16 S rDNA primers and the unbiased assessment of thermophile diversity.},
  author={Gillian C. Baker and Don Arthur Cowan},
  journal={Biochemical Society transactions},
  year={2004},
  volume={32 Pt 2},
  pages={218-21}
}
Our understanding of thermophile diversity is based predominantly on PCR studies of community DNA. "Universal" and domain-specific rRNA gene PCR primers have historically been used for the assessment of microbial diversity without adequate regard to the degree of specificity of primer pairs to different prokaryotic groups. In a reassessment of the published primers commonly used for "universal" and archaeal 16 S rDNA sequence amplification we note that substantial variations in specificity… CONTINUE READING

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