15-lipoxygenase metabolite(s) of arachidonic acid mediates adrenocorticotropin action in bovine adrenal steroidogenesis.

  title={15-lipoxygenase metabolite(s) of arachidonic acid mediates adrenocorticotropin action in bovine adrenal steroidogenesis.},
  author={Takeshi Yamazaki and Kiyoshi Higuchi and Shiro Kominami and Shigeki Takemori},
  volume={137 7},
The acute activation of adrenal glucocorticoid synthesis by ACTH has long been believed to be mediated by cAMP as the major second messenger, although increases in cellular cAMP concentration have not been observed at low concentrations of ACTH. We found that steroidogenesis in bovine adrenal fasciculata-reticularis cells was activated by the addition of arachidonic acid or its 15-lipoxygenase metabolite, 15-hydroperoxyeicosatetraenoic acid. The cellular 15-lipoxygenase pathway was… 
Role of Arachidonic Acid Metabolism in Acth-Stimulated Cortisol Secretion by Bovine Adrenocortical Cells
A StAR protein-independent role for the LPX and COX pathways in acute cortisol secretion is suggested and the hypothesis that LPX products of AA metabolism are key cellular signals when bovine ZF cells are acutely stimulated by physiological concentrations of ACTH is supported.
The role of arachidonic acid on LH-stimulated steroidogenesis and steroidogenic acute regulatory protein accumulation in MA-10 mouse leydig tumor cells
The results suggest that the effect of AA on LH- and cAMP-stimulated steroidogenesis is associated with the modulation of StAR protein expression.
The arachidonate 12/15 lipoxygenases
  • D. Conrad
  • Biology
    Clinical reviews in allergy & immunology
  • 1999
Summary12/15-Lipoxygenase is a highly regulated lipid-peroxidating enzyme whose expression and arachidonic acid metabolites are implicated in several important inflammatory conditions including
Arachidonic acid release from rat Leydig cells: the involvement of G protein, phospholipase A2 and regulation of cAMP production.
Results presented here suggest an involvement of PLA(2) and G proteins in the release of AA from hCG-stimulated Leydig cells, and under particular conditions, regulation of cAMP production by this fatty acid in these cells.
Concentration-dependent, biphasic effect of prostaglandins on avian corticosteroidogenesis in vitro.
PGs may be important modulators of corticosteroidogenesis in the avian adrenal gland, and the results suggest that the steroidogenic actions of PGs and ACTH converge on the same pool of steroidogenic enzymes leading to corticosterone.
Corticosterone enhances adrenocorticotropin-induced calcium signals in bovine adrenocortical cells.
Taken together, corticosterone secreted from adrenocortical cells activates ACTH-induced Ca(2+) signals and steroidogenesis by nongenomic means by acting on a target in the plasma membrane.
Suppression of lipid peroxidation in adrenal microsomes following ACTH administration to guinea pigs.
The results indicate that ACTH has a role in the regulation of adrenal LP, and the actions of ACTH to inhibit LP may contribute to an increase in adrenal hormone production by protecting steroidogenic enzymes from peroxidative degradation.


Role of lipoxygenase metabolites of arachidonic acid in the regulation of adrenocorticotropin secretion by perifused rat anterior pituitary cells.
The results suggested that epoxygenase metabolites may have a minor stimulatory effect on ACTH secretion and cyclooxygenases metabolites mayHave a minor inhibitory effect onACTH secretion, and the three major lipoxygen enzyme metabolites tested, 5(S), 12(S)-, and 15(S-hydroxyeicosatetraenoic acid (HETE), all stimulated sustained ACTH release in a dose-dependent manner.
Adrenal synthesis of corticosterone in response to ACTH in rats is influenced by leukotriene A4 and by lipoxygenase intermediates.
The studies suggest that the lipoxygenase pathway may be involved in ACTH-stimulated corticosterone synthesis.
Calcium ion as "second messenger" in corticoidogenic action of ACTH.
It was suggested that Ca2+ could regulate corticoidogenesis as a primary "second messenger" of ACTH through biosynthesis of so-called steroidogenic protein.
Distinctive properties of adrenal cortex mitochondria.
Regulatory role of guanosine 3',5'-monophosphate in adrenocorticotropin hormone-induced steroidogenesis.
It is suggested that cyclic GMP, rather than cyclic AMP, is the physiological mediator of adrenocorticotropic hormone-induced adrenal steroidogenesis.
Cyclic AMP-dependent protein kinase controls basal gene activity and steroidogenesis in Y1 adrenal tumor cells.
The dominant role played by cAMP-dependentprotein kinase in these adrenal cells was demonstrated by experiments showing the regulation of ornithine decarboxylase gene expression by protein kinase C requires basal camp-dependent protein Kinase activity.