13C labeling dynamics of intra- and extracellular metabolites in CHO suspension cells

Abstract

Materials and methods Experimental set-up CHO-K1 cells were cultivated in protein free TC-42 medium (TeutoCell, Bielefeld, Germany) in 250 ml baffled shake flasks. For the non-stationary experiment the cultures were inoculated at a start cell density of 2 × 10 cells/ml in a start volume of 120 ml. Four parallel cultivations were performed, two with 100% [U… (More)

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Cite this paper

@inproceedings{Wahrheit201313CLD, title={13C labeling dynamics of intra- and extracellular metabolites in CHO suspension cells}, author={Judith Wahrheit and Averina Nicolae and Elmar Heinzle}, year={2013} }