13C-carrier DNA shortens the incubation time needed to detect benzoate-utilizing denitrifying bacteria by stable-isotope probing.

@article{Gallagher200513CcarrierDS,
  title={13C-carrier DNA shortens the incubation time needed to detect benzoate-utilizing denitrifying bacteria by stable-isotope probing.},
  author={Evan Gallagher and Lora M. McGuinness and Craig D. Phelps and Lily Y. Young and Lee J Kerkhof},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 9},
  pages={5192-6}
}
The active bacterial community able to utilize benzoate under denitrifying conditions was elucidated in two coastal sediments using stable-isotope probing (SIP) and nosZ gene amplification. The SIP method employed samples from Norfolk Harbor, Virginia, and a Long-Term Ecosystem Observatory (no. 15) off the coast of Tuckerton, New Jersey. The SIP method was modified by use of archaeal carrier DNA in the density gradient separation. The carrier DNA significantly reduced the incubation time… CONTINUE READING

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