13C-NMR analysis of glucose metabolism during citric acid production by Aspergillus niger

@article{Peksel200113CNMRAO,
  title={13C-NMR analysis of glucose metabolism during citric acid production by Aspergillus niger},
  author={Aysegul Peksel and Nestor Torres and Juan Liu and G. Juneau and Christian Peter Kubicek},
  journal={Applied Microbiology and Biotechnology},
  year={2001},
  volume={58},
  pages={157-163}
}
The effect of glucose concentration on glycolytic metabolism under conditions of citric acid accumulation by Aspergillus niger was studied with 13C-labelled glucose. The results show that during cultivation at high glucose (14%, w/v), most of the label in citric acid is in C-2/C-4, and is thus due to the pyruvate carboxylase reaction. However, a significant portion is also present in C-1/C-5, whose origin is less clear but most likely due to reconsumption of glycerol and erythritol. Formation… CONTINUE READING