118 Purine De Novo Synthesis and Inosinic Branch Point in Vivo in Different Tissues. a Biomathematical Model

Abstract

We have elaborated a biomatheraatical model of purine de novo synthesis in vivo, which is visualized as a series of monomolecular reactions, leading from formate to IMP, from IMP either to AMP or to GMP:The three most important steps in this sequence are evaluated in terms of apparent rate constants (k1-k2-k3) through a system of differential equations, which includes the concentration and the specific radioactivity of the nucleotides after [14C] formate administration. Thus, we were able to follow (1) the rate of purine de novo synthesis in vivo in different rat organs (liver, kidney, spleen, heart), levator ani muscle, gastrocnemius and extensor digitorum longus, (2) the channeling of IMP into GMP or AMP (the “inosinic branch point”). Formation of IMP was most rapid in spleen, very fast in kidney, liver, heart, and lower in the muscles. The conversion of IMP into AMP was preferential in all tissues, an unexpectedly high rate of reaction IMP →-GMP was evident in the heart.

DOI: 10.1203/00006450-198807000-00142

Cite this paper

@article{Pizzichini1988118PD, title={118 Purine De Novo Synthesis and Inosinic Branch Point in Vivo in Different Tissues. a Biomathematical Model}, author={Maria Pizzichini and Anna Luisa Di Stefano and Germano Resconi and Enrico Marinello}, journal={Pediatric Research}, year={1988}, volume={24}, pages={130-130} }