11-cis-retinal reduces constitutive opsin phosphorylation and improves quantum catch in retinoid-deficient mouse rod photoreceptors.

@article{Ablonczy200211cisretinalRC,
  title={11-cis-retinal reduces constitutive opsin phosphorylation and improves quantum catch in retinoid-deficient mouse rod photoreceptors.},
  author={Zsolt Ablonczy and Rosalie K. Crouch and Patrice W. Goletz and T Michael Redmond and Daniel Knapp and Jian-xing Ma and Barbel Rohrer},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 43},
  pages={40491-8}
}
Rpe65(-/-) mice produce minimal amounts of 11-cis-retinal, the ligand necessary for the formation of photosensitive visual pigments. Therefore, the apoprotein opsin in these animals has not been exposed to its normal ligand. The Rpe65(-/-) mice contain less than 0.1% of wild type levels of rhodopsin. Mass spectrometric analysis of opsin from Rpe65(-/-) mice revealed unusually high levels of phosphorylation in dark-adapted mice but no other structural alterations. Single flash and flicker… CONTINUE READING