100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies

@article{Robinson1987100kDCV,
  title={100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies},
  author={Margaret S Robinson},
  journal={The Journal of Cell Biology},
  year={1987},
  volume={104},
  pages={887 - 895}
}
Proteins with molecular weights of around 100,000 (designated 100K) are found in all coated vesicles. Five monoclonal antibodies have been raised against the major 100K proteins of bovine brain coated vesicles, which migrate on SDS gels as three closely spaced bands. One antibody stains the middle band (band B), two stain both upper and lower bands (bands A and C), and two stain the lower band (band C) only. Thus, the polypeptides in bands A and C are related (but not identical), a result… CONTINUE READING
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