1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants.

@article{Stiel200718AB,
  title={1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants.},
  author={Andre C Stiel and Simon Trowitzsch and Gert Weber and Martin Andresen and Christian Eggeling and Stefan W Hell and Stefan Jakobs and Markus C Wahl},
  journal={The Biochemical journal},
  year={2007},
  volume={402 1},
  pages={35-42}
}
RSFPs (reversibly switchable fluorescent proteins) may be repeatedly converted between a fluorescent and a non-fluorescent state by irradiation and have attracted widespread interest for many new applications. The RSFP Dronpa may be switched with blue light from a fluorescent state into a non-fluorescent state, and back again with UV light. To obtain insight into the underlying molecular mechanism of this switching, we have determined the crystal structure of the fluorescent equilibrium state… CONTINUE READING