1-Million droplet array with wide-field fluorescence imaging for digital PCR.

@article{Hatch20111MillionDA,
  title={1-Million droplet array with wide-field fluorescence imaging for digital PCR.},
  author={A. Hatch and J. Fisher and A. Tovar and A. Hsieh and Robert Lin and S. Pentoney and D. L. Yang and A. Lee},
  journal={Lab on a chip},
  year={2011},
  volume={11 22},
  pages={
          3838-45
        }
}
Digital droplet reactors are useful as chemical and biological containers to discretize reagents into picolitre or nanolitre volumes for analysis of single cells, organisms, or molecules. However, most DNA based assays require processing of samples on the order of tens of microlitres and contain as few as one to as many as millions of fragments to be detected. Presented in this work is a droplet microfluidic platform and fluorescence imaging setup designed to better meet the needs of the high… Expand
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Digital droplet PCR on disk.
  • 58
  • PDF
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5
...

References

SHOWING 1-10 OF 63 REFERENCES
On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets.
  • 282
  • PDF
High-throughput quantitative polymerase chain reaction in picoliter droplets.
  • 294
  • PDF
On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets.
  • 175
  • PDF
Droplet microfluidic technology for single-cell high-throughput screening
  • 839
  • PDF
Spinning disk platform for microfluidic digital polymerase chain reaction.
  • 110
  • PDF
Multiplexed real-time polymerase chain reaction on a digital microfluidic platform.
  • 188
...
1
2
3
4
5
...