1 Keynote address by Nobel Laureate Phillip A. Sharp the 21st century opened with a revolution in RNA biology

  title={1 Keynote address by Nobel Laureate Phillip A. Sharp the 21st century opened with a revolution in RNA biology},
  author={Phillip A. Sharp},
  journal={Journal of Biomolecular Structure and Dynamics},
  pages={1 - 1}
  • P. Sharp
  • Published 18 May 2015
  • Physics
  • Journal of Biomolecular Structure and Dynamics
1 Keynote address by Nobel Laureate Phillip A. Sharp the 21st century opened with a revolution in RNA biology 


Synthetic oligodeoxynucleotide purification by polymerization of failure sequences.
Synthetic oligodeoxynucleotide is purified by capping failure sequences with an acrylated phosphoramidite followed by polymerization and product extraction. The method is suitable for large scale
Structures of the RNA-guided surveillance complex from a bacterial immune system
Cryo-electron microscopy is used to determine the subnanometre structures of Cascade before and after binding to a target sequence and reveal a sea-horse-shaped architecture in which the crRNAs are displayed along a helical arrangement of protein subunits that protect the crRNA from degradation while maintaining its availability for base pairing.
Flipped out: structure-guided design of selective pyrazolylpyrrole ERK inhibitors.
A novel series of pyrazolylpyrroles are identified as inhibitors of ERK, and structure-guided optimization culminated in the discovery of 6p, a potent and selective inhibitor ofERK.
Crystal structure of a CRISPR RNA–guided surveillance complex bound to a ssDNA target
The crystal structure of Cascade bound to a single-stranded DNA (ssDNA) target reveals that the CRISPR RNA and target strands do not form a double helix but instead adopt an underwound ribbon-like structure.
The structural biology of type I viral membrane fusion
The understanding of the structural transitions that are involved in this fusion pathway is reviewed, it is compared to the understanding of influenza virus membrane fusion, and the implications for retroviral membrane fusion are discussed.
Design of a G-quadruplex topology through glycosidic bond angles.
A rational approach is utilized for the design of G quadruplexes based on the position of the glycosidic bond angle to realize a programmed build up of DNA objects, devices, and materials.
Solution X-ray scattering combined with computational modeling reveals multiple conformations of covalently bound ubiquitin on PCNA
Two mutations originally identified in genetic screens and known to interfere with TLS are positioned directly beneath the bound ubiquitin in the alternative models, indicating that Ub–PCNA dynamically associated with PCNA and is capable of transitioning between a few discrete sites on the PCNA surface.
Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli
The structure of the Cascade complex provides novel molecular details of protein–protein and protein–RNA alignments and interactions required for generation of a complex mediating RNA-guided immune surveillance and explains why the seed sequence has a critical role in target DNA recognition.