1 Identification of lead-like and non-lead-like inhibitors for dengue virus NS 2 B / NS 3 protease by tryptophan fluorescence

In drug discovery, the occurrence of false positives is a major hurdle in the search for lead compounds that can be developed into drugs. A small-molecular-weight compound that inhibits dengue virus protease at low micromolar levels was identified in a screening campaign. Binding to the enzyme was confirmed by isothermal titration calorimetry (ITC) and… CONTINUE READING