1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome.

@article{Guo20051DEMA,
  title={1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome.},
  author={Yurong Guo and S Ma and Dmitry A. Grigoryev and Jennifer van Eyk and Joe Gn Garcia},
  journal={Proteomics},
  year={2005},
  volume={5 17},
  pages={4608-24}
}
Bronchoalveolar lavage fluid (BALF) is a complex mixture of proteins, which represents a unique clinically useful sampling of the lower respiratory tract. Many proteomic technologies can be used to characterize complex biological mixtures; however, it is not yet clear which technology(s) provide more information regarding the number of proteins identified and sequence coverage. In this study, we initially compared two common proteomic approaches, 2-D LC microESI MS/MS and 1-DE followed by gel… CONTINUE READING

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