1,25-Dihydroxycholecalciferol: Identification of the Proposed Active Form of Vitamin D3 in the Intestine

@article{Norman1971125DihydroxycholecalciferolIO,
  title={1,25-Dihydroxycholecalciferol: Identification of the Proposed Active Form of Vitamin D3 in the Intestine},
  author={Anthony W. Norman and James F. Myrtle and Ronald J. Miogett and H. George Nowicki and Vincent P. Williams and G. Popja�k},
  journal={Science},
  year={1971},
  volume={173},
  pages={51 - 54}
}
The major polar metabolite of cholecalciferol (vitamin D3) present in chick intestinal mucosa has been chemically characterized by mass spectrometric analysis to have a molecular formula of C27H4403 and a structure of 1,25-dihydroxycholecalciferol. This compound, which is produced in the kidney from 25-hydroxycholecalciferol, has been previously shown to be from 4 to 13 times as active as cholecalciferol in stimulating intestinal calcium transport. 1,25-Dihydroxycholecalciferol (previously… 
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The metabolism and mechanism of action of 1,25-dihydroxyvitamin D3.

  • R. Kumar
  • Biology, Medicine
    Kidney international
  • 1986
The tissue (intestinal tissue) is easily isolated and manipulated and hence, this is an ideal tissue in which to examine the mechanism of divalent cation transport and should help in understanding sterol hormone action.

1,25-Dihydroxycholecalciferol--a hormonally active form of vitamin D.

Calcinogenic factor in Solanum malacoxylon: evidence that it is 1,25-dihydroxyvitamin D3-glycoside.

1,25-dihydroxyvitamin D3, the active form of vitamin D, exists in the plant world, and its presence probably accounts for pathologic calcification in grazing animals ingesting Solanum malacoxylon.

Biologic effects of 1,25-dihydroxycholecalciferol (a highly active vitamin D metabolite) in acutely uremic rats.

The results indicate that renal conversion of calciferol to a more biologically active form is necessary for the stimulation of intestinal calcium absorption and calcium mobilization from bone, and that 1,25-diOH-CC may bypass a possible defect in vitamin D metabolism in uremia.

Vitamin D 3 -25-hydroxylase: tissue occurrence and apparent lack of regulation.

Effects of 1,25-dihydroxycholecalciferol on intestinal calcium transport in cortisone-treated rats.

The results support the concept that under the conditions of these experiments in the rat the apparent antagonism between glucocorticoids and vitamin D may be due to steroid hormone-related alterations in end organ function that are independent of any direct interaction between the hormone and the vitamin and that cannot be reversed by the vitamin.

Characterization of the metabolites of vitamin D 3 in the chick.

Influence of disodium ethane-1-hydroxy-1,1-diphosphonate on vitamin D metabolism in rats

The change in the renal metabolism of vitamin D in rats treated with a rachitogenic dose of EHDP may be caused by the modifications of the calcium metabolism brought about by the diphosphonate.

Studies on calciferol metabolism. 3. Comparison of species distribution and chromatographic separation of vitamin D metabolites.

The presence of metabolites 25-OH-CC and 4B, produced in vivo from vitamin D 3, was detected in chick skeleton, laying hen uterus, and the intestinal mucosa of the chick, rat, rabbit, frog, and monkey.

Synthetic Analogs of 1α,25-Dihydroxyvitamin D3 and Their Biological Activity

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References

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Identification of 1,25-dihydroxycholecalciferol, a form of vitamin D3 metabolically active in the intestine.

A biologically active vitamin D(3) metabolite ("peak V metabolite") more polar than 25-hydroxycholecalciferol has been isolated from chicken intestines in pure form as a mono(trimethylsilyl)ether…

In vitro production of 25-hydroxycholecalciferol.

  • M. HorstingH. DeLuca
  • Biology, Computer Science
    Biochemical and biophysical research communications
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Studies on calciferol metabolism. I. Production of vitamin D metabolite 4B from 25-OH-cholecalciferol by kidney homogenates.

Unique Biosynthesis by Kidney of a Biologically Active Vitamin D Metabolite

This work has shown that the tritium deficiency and the greater polarity are compatible with oxygen insertion at carbon 1 of 25-HCC, the major form of the circulating vitamin in blood plasma.

Identification of 1,25-Dihydroxycholecalciferol, a New Kidney Hormone controlling Calcium Metabolism

A vitamin D metabolite, 25-dihydroxycholecalciferol, is further hydroxylated by kidney before acting as a hormone on target tissues. The structure of this kidney metabolite is now described.

A rapidly acting metabolite of vitamin D3.

A vitamin D(3) metabolite in intestine more polar than 25-hydroxy-vitamin D(3)(25-OH D(3)) has been detected by countercurrent distribution. The intestinal metabolite is found also after…

Evidence for the biologically active form of cholecalciferol in the intestine.

The kinetics of the appearance of Metabolite 4B in the intestine and its binding to intestinal chromatin are consistent with the lag in the physiological response to cholecalciferol.

Vitamin D: A Cholecalciferol Metabolite Highly Active in Promoting Intestinal Calcium Transport

The polar intestinal metabolite greatly shortens this lag, stimulating maximum calcium transport by 9 hours and is a likely candidate for the biologically active form of cholecalciferol in the intestine.

Chromosomal Receptor for A Vitamin D Metabolite

Characterization of the still crude receptor fraction showed that it contains significant amounts of RNA and that it may exist in multiple forms, i.e., a 50,000-70,000 and a >200,000 molecular weight species.

The association of a metabolite of vitamin D3 with intestinal mucosa chromatin in vivo.