(−)-Epigallocatechin gallate, a polyphenolic tea antioxidant, inhibits peroxynitritemediated formation of 8-oxodeoxyguanosine and 3-nitrotyrosine

@article{Fiala2005EpigallocatechinGA,
  title={(−)-Epigallocatechin gallate, a polyphenolic tea antioxidant, inhibits peroxynitritemediated formation of 8-oxodeoxyguanosine and 3-nitrotyrosine},
  author={Emerich S. Fiala and Rama S. Sodum and M. Bhattacharya and H. Li},
  journal={Experientia},
  year={2005},
  volume={52},
  pages={922-926}
}
Reaction with peroxynitrite at pH 7.4 and 37°C was found to increase the 8-oxodeoxyguanosine levels in calf thymus DNA 35-38-fold. This oxidation of deoxyguanosine, as well as the peroxynitrite-mediated nitration of tyrosine to 3-nitrotyrosine, was significantly inhibited by ascorbic acid, glutathione and (−)-epigallocatechin gallate, a polyphenolic antioxidant present in tea. For 50% inhibition of the oxidation of deoxyguanosine to 8-oxodeoxyguanosine, 1.1, 7.6 of 0.25 mM ascorbate… Expand
Interaction of dicaffeoylquinic derivatives with peroxynitrite and other reactive nitrogen species.
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The assayed phenolics exert varying degrees of protection against the chemical modifications induced by RNS depending not only on the hydroxyl pattern, but also on the presence of bicarbonate. Expand
Comparative studies of the antioxidant effects of a naturally occurring resveratrol analogue – trans-3,3′,5,5′-tetrahydroxy-4′-methoxystilbene and resveratrol – against oxidation and nitration of biomolecules in blood platelets
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In vitro trans-3,3′, 5,5,5′-tetrahydroxy-4′-methoxystilbene and resveratrol have very similar protective effects against peroxynitrite-induced oxidative/nitrative damage to the human platelet proteins and lipids. Expand
Protective effects of resveratrol against oxidative/nitrative modifications of plasma proteins and lipids exposed to peroxynitrite.
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In vitro resveratrol, like well-known antioxidant DFO, has inhibitory effects on ONOO- -mediated oxidation of proteins and lipids in human plasma. Expand
Protective action of proanthocyanidin fraction from Medemia argun nuts against oxidative/nitrative damages of blood platelet and plasma components
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The preincubation of blood platelets and plasma with proanthocyanidin fraction from M. argun nuts reduced the formation of 3-nitrotyrosine, diminished oxidation of thiol groups, and decreased the level of carbonyl groups in proteins caused by 100 µM peroxynitrite. Expand
Epicatechin Inhibits Human Plasma Lipid Peroxidation Caused by Haloperidol In Vitro
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The presented results indicate that epicatechin—the major polyphenolic component of green tea reduced significantly human plasma lipid peroxidation caused by haloperidol, and was found to be a more effective antioxidant, than the solution of pure resveratrol or quercetin. Expand
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The presented in vitro results demonstrated for the first time that (-)-epicatechin might have protective effects against the impairment of structure and properties of Fg, caused by action of the strong biologic oxidant/nitration and inflammatory mediators. Expand
Effect of Tetrahydrobiopterin on Copper-Mediated DNA Oxidation: Its Prooxidant Property
Tetrahydrobiopterin (BH4) prevented an increase in the formation of 8-hydroxydeoxyguanosine (8-OHdG) in calf thymus DNA by the peroxynitrite (ONOO-) or Fe2+ + hydrogen peroxide (H2O2) system. But,Expand
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The obtained results demonstrate that the compounds from herbal plants, including A. millefolium, A. montana, E. purpurea, C. recutita, S. virgaurea, possess antioxidative properties and protect platelet proteins against peroxynitrite toxicity in vitro, similar to the glycoconjugates from C. canadensis. Expand
Antioxidant and antiaggregatory effects of an extract from Conyza canadensis on blood platelets in vitro
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The present study suggests that the natural polysaccharide extract from Conyza canadensis has antiaggregatory and antioxidative activities, and therefore may be beneficial in the prevention of peroxynitrite-related diseases, such as cardiovascular or inflammatory diseases. Expand
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