Corpus ID: 173106954

神经肽Y Y5受体基因反义寡核苷酸脑室给药对大鼠血清瘦素、胰岛素及TNF-α水平的影响

@inproceedings{2009YY,
  title={神经肽Y Y5受体基因反义寡核苷酸脑室给药对大鼠血清瘦素、胰岛素及TNF-α水平的影响},
  author={解建波},
  year={2009}
}
  • 解建波
  • Published 2009
  • 目的观察神经肽YY5(NPYY5)受体基因反义寡核苷酸脑室给药对伴糖尿病缺血再灌注(I/R)大鼠血清瘦素、胰岛素与TNF-α水平的影响。方法链脲佐菌素空腹腹腔注射制备糖尿病大鼠模型,线栓法闭塞大脑中动脉制作脑I/R大鼠模型;治疗组经导管向脑室注入50μg(5μg/μl)NPY Y5受体基因反义寡核苷酸,每天3次给药,连续用药3d;采用ELISA双抗体夹心法测定血清TNF-α与瘦素含量,放射免疫法测定胰岛素含量。结果I/R损伤后,大鼠血清瘦素、胰岛素、TNF-α水平较对照组显著升高(P〈0.05,P〈0.01);NPYY5受体基因反义脱氧核苷酸侧脑室注射干预后,其血清瘦素、胰岛素水平明显下降(P〈0.05),TNF-α水平显著降低(P〈0.01)。结论神经肽YY5受体基因反义寡核苷酸侧脑室给药可降低伴糖尿病I/R大鼠的血清瘦素、TNF-α与胰岛素水平,改善外周瘦素抵抗与胰岛素抵抗(IR),促进脑梗死恢复。 

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