基于氨基化SiO 2 纳米颗粒的新型基因载体

@article{2002SiO2,
  title={基于氨基化SiO 2 纳米颗粒的新型基因载体},
  author={何晓晓 and 王柯敏 and 谭蔚泓 and 刘斌 and 林霞 and 黄杉生 and 李杜 and 何春梅 and 李军},
  journal={Chinese Science Bulletin},
  year={2002},
  volume={47},
  pages={1365-1369}
}
采用正硅酸乙酯与N-( β -氨乙基)- γ -氨丙基三乙氧基硅烷在油包水形成的微胶囊中同步水解的方法, 制备了大小均匀的氨基化二氧化硅纳米颗粒. 由于表面氨基化使纳米颗粒在一定的pH环境下的 x (zeta)电位为正值, 与带负电荷的质粒DNA通过静电结合能快速形成纳米颗粒-质粒DNA复合物, 并对结合的DNA有明显的保护作用. 结合纳米技术、生物技术与基因工程技术, 构建了基于氨基化SiO 2 纳米颗粒的新型非病毒型基因载体, 应用这一新型基因载体成功地将绿色荧光蛋白(green fluorescence protein, GFP)表达载体pIRGFP导入COS-7细胞, 并在细胞内产生高水平的绿色荧光蛋白表达. 
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