„Natural”︁ killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell

@article{Kiessling1975NaturalKC,
  title={„Natural”︁ killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell},
  author={Rolf Kiessling and Eva Klein and Hugh F. Pross and Hans Wigzell},
  journal={European Journal of Immunology},
  year={1975},
  volume={5}
}
Normal mice contain cytolytic cells with specificity for in vitro grown mouse Moloney leukemia cells. Such killer cells are most frequent in the spleens; lymph node and bone marrow contain less and thymus virtually no killer activity. Peak activity is found around one to three months of age. Spleen cells from genetically athymic mice are as active killer cells as those from normal mice of the same strain. Treatment with anti‐theta serum plus complement followed by removal of adherent and… 
„Natural”︁ killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype
TLDR
Analysis of the kinetics of the cytotoxic assay revealed a rapid induction of lysis within one to four hours, arguing against any conventional in vitro induction of immune response.
Natural cytotoxicity to human leukemia mediated by mouse non‐t cells
TLDR
It is shown that mouse NK cells also function in a xenogeneic system, and unlabelled mouse T lymphoma YAC‐1 cells were efficient competitors for lysis of human T leukemia lines.
Killer cells: a functional comparison between natural, immune T-cell and antibody-dependent in vitro systems
TLDR
The present results further distinguish the NK cell from cytotoxic T lymphocytes or from antibody- dependent killer cells.
Surface markers on natural killer cells of the mouse
TLDR
The results indicate that the absorbed anti‐BAT can distinguish NK cells from other known subsets of T and B cells.
Biology of Natural Killer Cells
Natural killer cell activity in reticulum‐cell sarcomas of SJL/J mice. II. Analysis of RCS‐associated NK activity
TLDR
The data indicate that the RCS tumors represent either neoplastic expansion of conventional NK cells with somewhat modified properties, or else an entirely new class of cytotoxic effector cells.
Hybrid resistance to EL-4 lymphoma cells. I. Characterization of natural killer cells that lyse EL-4 cells and their distinction from marrow-dependent natural killer cells
TLDR
It is concluded that Hh-1b is the common antigen present in EL-4 and YAC-1 cells, because B6D2F1 anti-B6 (anti- Hh -1b) cytotoxic T lymphocytes lysed both the tumor cells.
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References

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„Natural”︁ killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype
TLDR
Analysis of the kinetics of the cytotoxic assay revealed a rapid induction of lysis within one to four hours, arguing against any conventional in vitro induction of immune response.
Specific in vitro Cytotoxicity of Thymus-derived Lymphocytes sensitized to Alloantigens
TLDR
This study tested the effect of anti-θ serum on the cytotoxic activity of spleen cells sensitized to alloantigens, and its effect on alloantsibody-producing cells present in the same spleen cell populations was tested in parallel.
Antitumour activity in vitro dependent on immune B lymphocytes.
TLDR
Fractionation of the lymphocytes in this system has shown the active effector cells to be both thymus processed lymphocytes (T cells)3 and non-T cells3,4.
CELLS MEDIATING SPECIFIC IN VITRO CYTOTOXICITY
TLDR
It is suggested that only T cells are involved in the killing of target cells by allogeneic immune cells in vitro, in this system, andceptor-bearing cells involved in killing in the present system are thus very probably T cells.
Separation of Cells According to Surface Antigens by the Use of Antibody‐Coated Columns. Fractionation of Cells Carrying Immunoglobulins and Blood Group Antigen
TLDR
It has been found possible to separate cells according to their surface anti gens by the use of antibody‐coated columns and high‐avidity immune antibodies were found to be more efficient than ‘natural’ anti‐A antibodies in this test.
Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells.
TLDR
Results obtained support the concept of a cell-associated antigen-specific receptor being present on the outer surface of immune cells, displaying the same antigen-binding specificity as the potential product of the cell, the humoral antibody.
Characterization of the antibody-dependent cytotoxic cell. A non-phagocytic monocyte?
TLDR
The cytotoxic effector cell showed similar characteristics to monocytes with respect to size, surface adherence properties and binding affinity for immunoglobulin subclasses.